Landon C. Brown1, Susan Halabi1,2, Joseph D. Schonhoft3, Qian Yang2, Jun Luo4, David M. Nanus5, Paraskevi Giannakakou5, Russell Z. Szmulewitz6, Daniel C. Danila5,7, Ethan S. Barnett7, Emily A. Carbone7, Jimmy L. Zhao7, Patrick Healy8, Monika Anand1, Audrey Gill3, Adam Jendrisak3, William R. Berry1, Santosh Gupta9, Simon G. Gregory9, Richard Wenstrup3, Emmanuel S. Antonarakis10, Daniel J. George1, Howard I. Scher5,7, and Andrew J. Armstrong1
H.I. Scher a,b,,1, A.J. Armstrong c,*,1, J.D. Schonhoft d, A. Gill d, J.L. Zhao a, E. Barnett a, E. Carbone a, J.Lud, E.S. Antonarakis e, J. Luo e, S. Tagawa b, C.H. dos Anjos a, Q. Yang f, D. George c, R. Szmulewitz f,g, D.C. Danila a,b, R. Wenstrup d, M. Gonen a,2,
S. Halabi c,f,2
Joseph D. Schonhoft1, Jimmy L. Zhao2,3, Adam Jendrisak1, Emily A. Carbone4, Ethan S. Barnett4, Melanie A. Hullings4,5, Audrey Gill1, Ramsay Sutton1, Jerry Lee1, Angel E. Dago1, Mark Landers1, Samuel F. Bakhoum3,6, Yipeng Wang1, Mithat Gonen7, Ryan Dittamore1, and Howard I. Scher2,8
Andrew J. Armstrong, MD, ScM1; Jun Luo, PhD2; David M. Nanus, MD3; Paraskevi Giannakakou, PhD3; Russell Z. Szmulewitz, MD4; Daniel C. Danila, MD3,5; Patrick Healy, MS; Monika Anand, PhD1; William R. Berry, MD1; Tian Zhang, MD1; Michael R. Harrison, MD1; Changxue Lu, MM, PhD2; Yan Chen, BM, PhD2; Giuseppe Galletti, MD, PhD3; Joseph D. Schonhoft, PhD7; Howard I. Scher, MD5; Richard Wenstrup, MD7; Scott T. Tagawa, MD3; Emmanuel S. Antonarakis, MD2,8; Daniel J. George, MD1; and Susan Halabi, PhD8
Conteduca V, Ku SY, Puca L, Slade M, Fernandez L, Hess J, Bareja R, Vlachostergios PJ, Sigouros M, Mosquera JM, Sboner A, Nanus DM, Elemento O, Dittamore R, Tagawa ST, Beltran H.Mol Cancer Ther. 2020 May;19(5):1157-1164. DOI: 10.1158/1535-7163
Malihi PD, Graf RP, Rodriguez A, Ramesh N, Lee J, Sutton R, Jiles R, Ruiz Velasco C, Sei E, Kolatkar A, Logothetis C, Navin NE, Corn P, Aparicio AM, Dittamore R, Hicks J, Kuhn P, Zurita AJ.Clin Cancer Res. 2020 Apr 27. DOI: 10.1158/1078-0432.CCR-19-4100
Using nonenrichment-based, potentially more sensitive Epic Sciences circulating tumor cell (CTC) platform, we sought to detect and characterize CTCs in untreated, high-risk localized prostate cancer and to evaluate their clinical implication.
Delta-like protein 3 expression and therapeutic targeting in neuroendocrine prostate cancer. Science Translational Medicine 20 Mar 2019. DOI: 10.1126/scitranslmed.aav0891
Prospective Multicenter Validation of Androgen Receptor Splice Variant 7 and Hormone Therapy Resistance in High-Risk Castration-Resistant Prostate Cancer: The PROPHECY Study. Published online March 13, 2019. DOI: 10.1200/JCO.18.01731 Journal of Clinical Oncology.
The heterogeneity of an individual patient's tumor has been linked to treatment resistance, but quantitative biomarkers to rapidly and reproducibly evaluate heterogeneity in a clinical setting are currently lacking.
Androgen receptor (AR) is frequently detected in breast cancers, and AR-targeted therapies are showing activity in AR-positive (AR+) breast cancer. However, the role of AR in breast cancers is still not fully elucidated and the biology of AR in breast cancer remains incompletely understood.
The fetal oncogene 5T4 is a cell surface protein, with overexpression observed in a variety of cancers as compared to normal adult tissue. The ability to select patients with tumors that express high levels of 5T4 may enrich a clinical trial cohort with patients most likely to respond to 5T4 targeted therapy.
Abiraterone acetate (AA) inhibits androgen biosynthesis and prolongs survival in men with metastatic castration-resistant prostate cancer (mCRPC) when combined with prednisone (P). Resistance to therapy remains incompletely understood. In this open-label, single-arm, multicenter phase II study we investigated the clinical benefit of increasing the dose of AA at the time of resistance to standard-dose therapy.
The cancer community understands the value of blood profiling measurements in assessing and monitoring cancer. We describe an effort among academic, government, biotechnology, diagnostic, and pharmaceutical companies called the Blood Profiling Atlas in Cancer (BloodPAC) Project.
Scher, H et al. Nuclear-specific AR-V7 Protein Localization is Necessary to Guide Treatment Selection in Metastatic Castration-resistant Prostate Cancer. 2016 November. DOI:/10.1016/j.eururo.2016.11.024
Genomic instability is a hallmark of cancer often associated with poor patient outcome and resistance to targeted therapy. Assessment of genomic instability in bulk tumor or biopsy can be complicated due to sample availability, surrounding tissue contamination, or tumor heterogeneity.
Anantharaman, A et al. Programmed death-ligand 1 (PD-L1) characterization of circulating tumor cells (CTCs) in muscle invasive and metastatic bladder cancer patients. BMC Cancer 201616:744 DOI: 10.1186/s12885-016-2758-3
McDaniel AS, et al. Phenotypic diversity of circulating tumor cells in patients with metastatic castration-resistant prostate cancer. BJU Int. 2016 Aug . doi: 10.1111/bju.13631. [Epub ahead of print]
Lowes, LE et al. Circulating Tumor Cells (CTC) and Cell-Free DNA (cfDNA) Workshop 2016: Scientific Opportunities and Logistics for Cancer Clinical Trial Incorporation. Int J Mol Sci. 2016 Sep ;17(9)
Scher, H et al. Association of AR-V7 on Circulating Tumor Cells as a Treatment-Specific Biomarker With Outcomes and Survival in Castration-Resistant Prostate Cancer. JAMA Oncology. Advance Online Publication: 2016 June. DOI:10.1001/jamaoncol.2016.1828
Beltran, H et al. The Initial Detection and Partial Characterization of Circulating Tumor Cells in Neuroendocrine Prostate Cancer. 2016 March. DOI:10.1158/1078-0432.CCR-15-0137
Multiple myeloma (MM) remains an incurable disease despite recent therapeutic improvements. The ability to detect and characterize MM circulating tumour cells (CTCs) in peripheral blood provides an alternative to replace or augment invasive bone marrow (BM) biopsies with a simple blood draw, providing real-time, clinically relevant information leading to improved disease management and therapy selection.
Beltran, et al. (2015) The Initial Detection and Partial Characterization of Circulating Tumor Cells in Neuroendocrine Prostate Cancer. Clinical Cancer Research. Author Manuscript Published OnlineFirst on December 15, 2015; DOI: 10.1158/1078-0432.CCR-15-0137
Punnoose, et al. (2015) PTEN loss in circulating tumour cells correlates with PTEN loss in fresh tumour tissue from castration-resistant prostate cancer patients. British Journal of Cancer, 1–9 | doi: 10.1038/bjc.2015.332
Circulating tumor cells (CTCs) analysis is a promising new diagnostic field to estimate risk and monitor treatment efficacy, metastatic relapse, and progression in cancer patients. The study aim was to isolate and characterize CTCs in blood samples of Ewing sarcoma (ES) patients exploiting two main characteristics: CD99 expression and presence of chromosomal translocations.
Werner, et al (2015) Analytical validation and capabilities of the Epic CTC platform: enrichment-free circulating tumor cell detection and characterization. J circulating biomarkers 4:3. doi: 10.5772/60725